Journal: ACS chemical biology

Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators

doi: 10.1021/cb4004942

Figure Lengend Snippet: Binding of antibody from human serum to cells derivatized with synthetic antigens as quantified by flow cytometry (phycoerythrin channel, FL-2). (A) IgM bound. (B) IgG bound. (C) Competitive inhibition of IgG binding by soluble rhamnose. (D) IgG subtypes bound. Data points represent mean values of 3–8 experiments. Error bars represent 95% confidence intervals. T-test comparisons at 95% confidence were performed using GraphPad Prism 5.0 (** p < 0.01, *** p < 0.001).

Article Snippet: T-test comparisons at 95% confidence were performed using GraphPad Prism 5.0 (** p < 0.01, *** p < 0.001).

Techniques: Binding Assay, Flow Cytometry, Inhibition

Journal: ACS chemical biology

Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators

doi: 10.1021/cb4004942

Figure Lengend Snippet: Comparison of ligand-dependent cytotoxicity following labeling of CHO cells with synthetic antigens. Data points represent the mean values of 12 experiments. Error bars represent 95% confidence intervals. T-test comparisons at 95% confidence were performed using GraphPad Prism 5.0 (** p < 0.01, *** p < 0.001).

Article Snippet: T-test comparisons at 95% confidence were performed using GraphPad Prism 5.0 (** p < 0.01, *** p < 0.001).

Techniques: Labeling

Journal: ACS chemical biology

Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators

doi: 10.1021/cb4004942

Figure Lengend Snippet: Comparison of monovalent rhamnose antigen 3 to bivalent rhamnose antigen 5. (A) IgM recruited. (B) IgG recruited. (C) CDC assay. Data points represent mean values of 3 experiments. Error bars represent 95% confidence intervals. T-test comparisons at 95% confidence were performed using GraphPad Prism 5.0 (** p < 0.01, *** p < 0.001).

Article Snippet: T-test comparisons at 95% confidence were performed using GraphPad Prism 5.0 (** p < 0.01, *** p < 0.001).

Techniques: CDC Assay

Journal: ACS chemical biology

Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators

doi: 10.1021/cb4004942

Figure Lengend Snippet: Scatter plots showing correlations between data sets from Figure 6. Linear lines of best fit and Spearman rank correlation coefficients (rs) were calculated using GraphPad Prism 5.0.

Article Snippet: T-test comparisons at 95% confidence were performed using GraphPad Prism 5.0 (** p < 0.01, *** p < 0.001).

Techniques:

Journal: Journal of Virology

Article Title: Implications of the 375W mutation for HIV-1 tropism and vaccine development

doi: 10.1128/jvi.01522-23

Figure Lengend Snippet: IC50 values of V3 loop/glycan and V1V2 loop/glycan mAb neutralization. Geometric mean IC50s and 95% confidence intervals were calculated and plotted in GraphPad for PGT128 (A), 10-1074 (B), PGT145 (C), PG9 (D), and PG16 (E) mAbs. Two different neutralization assays were performed, and each experiment was made in duplicate. Confidence intervals for WT and 375W mutants are presented in blue or orange error bars, respectively. WT IC50s are represented by blue circles (⚫︎), and 375W mutant IC50s, by orange circles (⚫︎).

Article Snippet: Geometric mean IC50s and 95% confidence intervals were calculated and plotted in GraphPad for PGT128 ( A ), 10-1074 ( B ), PGT145 ( C ), PG9 ( D ), and PG16 ( E ) mAbs.

Techniques: Neutralization, Mutagenesis

Journal: Journal of Virology

Article Title: Implications of the 375W mutation for HIV-1 tropism and vaccine development

doi: 10.1128/jvi.01522-23

Figure Lengend Snippet: mAbs used in this study

Article Snippet: Geometric mean IC50s and 95% confidence intervals were calculated and plotted in GraphPad for PGT128 ( A ), 10-1074 ( B ), PGT145 ( C ), PG9 ( D ), and PG16 ( E ) mAbs.

Techniques: